Sandelisa90 (Talk | contribs) |
Sandelisa90 (Talk | contribs) |
||
| Line 2: | Line 2: | ||
'''Cell surface Display''' | '''Cell surface Display''' | ||
| - | ''Primers arrived:'' we did a PCR to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | + | ''Primers arrived:'' we did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. |
We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Primers arrived: we did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
"
