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Exeter/3 September 2013
From 2013.igem.org
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<div class="span" style="text-align:justify"> | <div class="span" style="text-align:justify"> | ||
| - | == Results of liquid cultures from yesterday == | + | == Results of liquid cultures from yesterday == __NOTOC__ |
| Line 51: | Line 51: | ||
! Part !! Did it work? | ! Part !! Did it work? | ||
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS A || ✓ |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS B || ✓ |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS C || ✓ |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS D || ✓ |
|- | |- | ||
| K592022 A || ✗ | | K592022 A || ✗ | ||
| Line 74: | Line 74: | ||
== Miniprepped == | == Miniprepped == | ||
| - | * | + | *<i>ompF</i> + RBS A |
| - | * | + | *<i>ompF</i> + RBS B |
| - | * | + | *<i>ompF</i> + RBS C |
| - | * | + | *<i>ompF</i> + RBS D |
*K592011 B | *K592011 B | ||
*gblocks 1 to 15 | *gblocks 1 to 15 | ||
| Line 117: | Line 117: | ||
| gBlock 15 || 87.0 | | gBlock 15 || 87.0 | ||
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS A || 41.2 |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS B || 57.8 |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS C || 74.2 |
|- | |- | ||
| - | | | + | | <i>ompF</i> + RBS D || 46.7 |
|- | |- | ||
| K592011 B || 159.0 | | K592011 B || 159.0 | ||
Revision as of 20:37, 1 October 2013
Results of liquid cultures from yesterday
gBlocks
| gBlock | Colour |
|---|---|
| 1 | No |
| 2 | No |
| 3 | Vibrant pink |
| 4 | No |
| 5 | Vibrant pink |
| 6 | No |
| 7 | Peach |
| 8 | No |
| 9 | Pink - not so vibrant |
| 10 | Vibrant pink |
| 11 | Vibrant pink |
| 12 | Pink - not so vibrant |
| 13 | Pink - not so vibrant |
| 14 | No |
| 15 | No |
| Part | Did it work? |
|---|---|
| ompF + RBS A | ✓ |
| ompF + RBS B | ✓ |
| ompF + RBS C | ✓ |
| ompF + RBS D | ✓ |
| K592022 A | ✗ |
| K592022 B | ✓ |
| K592011 A | ✗ |
| K592011 B | ✓ |
| K864404 | ✓ |
Miniprepped
- ompF + RBS A
- ompF + RBS B
- ompF + RBS C
- ompF + RBS D
- K592011 B
- gblocks 1 to 15
NanoDrop from miniprep
| Part | Concentration (ng/ul) |
|---|---|
| gBlock 1 | 27.4 |
| gBlock 2 | 28.6 |
| gBlock 3 | 101.2 |
| gBlock 4 | 37.2 |
| gBlock 5 | 168.6 |
| gBlock 6 | 21.3 |
| gBlock 7 | 153.7 |
| gBlock 8 | 30.8 |
| gBlock 9 | 247.1 |
| gBlock 10 | 183.4 |
| gBlock 11 | 197.4 |
| gBlock 12 | 105.1 |
| gBlock 13 | 126.6 |
| gBlock 14 | 172.5 |
| gBlock 15 | 87.0 |
| ompF + RBS A | 41.2 |
| ompF + RBS B | 57.8 |
| ompF + RBS C | 74.2 |
| ompF + RBS D | 46.7 |
| K592011 B | 159.0 |
Take me back to the notebook.
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