Team:EPF Lausanne/Calendar/15 September 2013

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Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.
Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.
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'''Nanoparticles'''
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<BR>- Digestion assays
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<BR>- Culture of MMP2 and gelE from Sensing group

Revision as of 21:27, 2 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Analysis of the sequencing resutls of the three promoters (sensing-module)
The sequencing results were rather promising except for the Hya promoter. The sample that I send did not contain it. So I purified one in order to send it again on Tuesday. This sample was from one of the assays. I send this sample because it did not turn green when I added arabinose indicating that the Arabinose inducible promoter had effectively been removed and hopefully replaced by the hya promoter.

Functional assays for the three promoters (sensing-module)
I again tried to grow the bacteria in an LB medium to which I added different amounts of either 10X MOPS or 10X HEPES buffer. But again the cultures did not turn green.


MinPrep of the hya-plasmid
Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.

Wiki Links


Nanoparticles
- Digestion assays
- Culture of MMP2 and gelE from Sensing group

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