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Team:UNITN-Trento/Notebook/Labposts/09/07
From 2013.igem.org
(Difference between revisions)
(Created page with "{ "date" : "2013-09-09", "author" : "gabriele", "title" : "Amplifications", "content" : "<html>I amplified the confirmed constructs (pLAC+SAMsynthetase and the other one with...") |
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{ | { | ||
| - | "date" : "2013-09- | + | "date" : "2013-09-11", |
"author" : "gabriele", | "author" : "gabriele", | ||
| - | "title" : " | + | "title" : "Cloning", |
| - | "content" : "<html>I | + | "content" : "<html>I treated the o/n digestion of mutB with SAP and of EFE with DpnI. I miniprepped yesterday inocula with bad results (I used a new kit) and screened EFE PCR, which was positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|PCR Screening|<html><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-20130911_EFEpcr.jpg\" /></html>}}<html>Then I ligated the purified O/N digestions: Ctrl, 1:1 and 1:2. I transformed the ligation in NEB10β and plated them.</html>", |
| - | "tags" : " | + | "tags" : "EFE-BlueLight" |
} | } | ||
Revision as of 09:23, 3 October 2013
{ "date" : "2013-09-11", "author" : "gabriele", "title" : "Cloning", "content" : "I treated the o/n digestion of mutB with SAP and of EFE with DpnI. I miniprepped yesterday inocula with bad results (I used a new kit) and screened EFE PCR, which was positive!
Then I ligated the purified O/N digestions: Ctrl, 1:1 and 1:2. I transformed the ligation in NEB10β and plated them.", "tags" : "EFE-BlueLight" }![](\"https://static.igem.org/mediawiki/2013/5/50/Tn-20130911_EFEpcr.jpg\")
