Team:UNITN-Trento/Notebook/Labposts/08/06

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Latest revision as of 09:43, 3 October 2013

{ "date" : "2013-08-02", "author" : "Michele", "title" : "Doing a mess", "content" : "So, I didn't believe Cristina when in July she told us: \"In August we're going to work twice than this\" but I had better trust her. Today I did two TLC on four different samples to detect MeSA presence.

TLC Image

As you can see on the image of one of the two TLC only the control (MeSA pure diluited 1:1 in CH2Cl2) ran. These was the third TLC that failed then we decided to avoid more TLC tests. During the day I checked also the growth of Bacillus (link previous post sporulation). In the crazy afternoon after an hard fight with the order in the Lab I performed a screening on GFP (Bba_E0840) to verify if our stock was correct.

Gel Image

As you can is present the band at 878 bp so the screening gave positive results. During the digestion and the electrophoretic run I also did a transforamtion of Neb10β with EFE in pSB1C3. ", "tags" : "GFP-EFE-TLC" }