Team:UNITN-Trento/Notebook/Labposts/07/71

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{"date" : "2013-07-30","author" : "thomas","title" : "Bacillus transformation!!!","content" : "<html>In order to test the transformation protocol for <i>B. subtilis</i> that Groeningen team kindly sent to us I prepared a competent cell Media following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation\">this protocol</a>. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following <a href=\"\">this protocol</a>. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/e/ec/Tn-2013_bacillus_transformation_plate_1.jpg\" style =\"width: 450px\"></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Bacillus microscope image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/6/65/Tn-2013_gram_staining_bacillus.jpg\" style =\"width: 450px\"></center></html>}}<html> As you can see from the pictures, seems that <i>Bacillus</i> was successful transformed!!!!</html>","tags" : "b. subtilis-pspac"}

Latest revision as of 10:48, 3 October 2013

{"date" : "2013-07-30","author" : "thomas","title" : "Bacillus transformation!!!","content" : "In order to test the transformation protocol for B. subtilis that Groeningen team kindly sent to us I prepared a competent cell Media following this protocol. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following this protocol.

Plate image
Bacillus microscope image
As you can see from the pictures, seems that Bacillus was successful transformed!!!!","tags" : "b. subtilis-pspac"}

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