Team:TU Darmstadt/labbook

From 2013.igem.org

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=== ''' Our Workflow ''' ===
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See how we worked on our [https://2013.igem.org/Team:TU_Darmstadt/labbook/DetectionConstruct detection construct]. See how we managed to work on our Biobricks [https://2013.igem.org/Team:TU_Darmstadt/labbook/Biobricks pSB1C3-LssmOrange and pSB1C3-mKate].
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<meta name="author" content="Anne Schieferdecker">
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<meta name="editor" content="html-editor phase 5">
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      <table border="1" rules="rows"  >
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        <tr>
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                <td>13.08</td>
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                <td>gBLOCK assembly of CMK and TLO</td>
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                <td></td>
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                <td><ul><li>PCR mixture
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                                <ul><li>1 µL of each gBLOCK
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                                <ul>
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                                        <li>CMK: B_01 - B_04</li>
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                                        <li>TLO: A_01 - A_06</li>
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                                </ul></li>
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                                <li>10 µL 5x Q5 Reaction Buffer</li>
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                                <li>2 µL dNTPs</li>
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                                <li>1 µL Q5 Hot Start Polymerase</li>
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                                <li>10 µL 5x Q5 High GC Enhancer</li>
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                                <li>1 µL primer suffix-R (10 mM)</li>
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                                <li>1 µL primer prefix_R (10 mM)</li>
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                        </ul></ul></li>
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                        <ul><li>PCR program (40 cycles)
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                                <ul><li>initial denaturation 94°C, 100s</li>
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                                <li>denaturation 94°C, 55s</li>
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                                <li>annealing 64°C, 55s</li>
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                                <li>elongation 72°C, 120s</li>
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                                <li>final elongation 72°C, 300s</li>
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                        </li></ul></ul>
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                        <ul><li>preparative 1% agarose gel
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                                <ul><li>gel displays bands of expected size, therefore assembly was successful</li>
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                        </ul></li></ul>
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                </td>
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                <td><img src="https://static.igem.org/mediawiki/2013/6/6c/Darmstadt13_InFusion_130813.png" alt=""</td>
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        </tr>
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Revision as of 11:43, 3 October 2013

Labbook | Protocols | Materials |

Our Workflow

See how we worked on our detection construct. See how we managed to work on our Biobricks pSB1C3-LssmOrange and pSB1C3-mKate.

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/labbook"