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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
'''Cell surface Display''' | '''Cell surface Display''' | ||
| + | Our Primers arrived today. <BR> | ||
| - | '' | + | ''PCR'' <BR> |
| - | We thus | + | We did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. |
| + | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
'''General''' | '''General''' | ||
PCR
We did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
