GigiGoesGugu (Talk | contribs) |
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We did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | We did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | ||
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
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'''General''' | '''General''' | ||
Competent cells try 2. | Competent cells try 2. | ||
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{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
PCR
We did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
