Team:EPF Lausanne/Calendar/29 July 2013

From 2013.igem.org

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'''Cell Surface Display'''<BR>
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<font size = "4">Cell Surface Display</font>
''PCR optimisation''<BR>
''PCR optimisation''<BR>
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A 0.8% Electrophoresis gel was performed to verify the reation's products.
A 0.8% Electrophoresis gel was performed to verify the reation's products.
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'''Nanoparticles'''<BR>
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<font size = "4">Nanoparticles</font>
''Making Nanoparticles, 1st try'' <BR>
''Making Nanoparticles, 1st try'' <BR>

Revision as of 14:41, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header


Cell Surface Display

PCR optimisation
The previous PCR we did to amplify pINP_construct was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C. A 0.8% Electrophoresis gel was performed to verify the reation's products.

Nanoparticles

Making Nanoparticles, 1st try
We added the cargo molecule (food dye) to GPs and left them for stirring (during 3 days)).