Team:TU Darmstadt/materials/Chloramphenicol

From 2013.igem.org

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== Ampicillin ==
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== Chloramphenicol ==
=== About ===
=== About ===
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Ampcillin is a beta-lactam antibiotic that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria.
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Chloramphenicol is a bacteriostatic [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.<sup>[1]</sup>
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=== Stock ===
=== Stock ===
# Mix:
# Mix:
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#* 4 g ampicillin (100 mg/mL)
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#* 1 g chloramphenicol (25 mg/mL)
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#* ad. 40 mL dist. water
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#* ad. 40 mL ethanol
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# sterile filtration
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# aliqout in 1 mL stocks and store at -20°C
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# aliquot in 1 mL stocks and store at -20°C
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=== Stability ===
=== Stability ===
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* Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
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* Chloramphenicol is not autoclavable, it has to be added before [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates pouring plates] (keep in mind that the agar is not too hot when adding it)
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* Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])
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=== References ===
=== References ===
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* http://en.wikipedia.org/wiki/Ampicillin
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http://en.wikipedia.org/wiki/Chloramphenicol
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Revision as of 17:19, 3 October 2013

Labbook | Protocols | Materials |

Contents

Chloramphenicol

About

Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.[1]

Stock

  1. Mix:
    • 1 g chloramphenicol (25 mg/mL)
    • ad. 40 mL ethanol
  2. aliqout in 1 mL stocks and store at -20°C

Stability

  • Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)

References

http://en.wikipedia.org/wiki/Chloramphenicol

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