GigiGoesGugu (Talk | contribs) |
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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | Sensing <BR> | + | <font size = "4"> Sensing </font> <BR> |
| - | + | ''Measuring GFP expression'' <BR> | |
| - | Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter. | + | -Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter. |
This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc. | This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc. | ||
The absorbance was measured in a plate reader during 18h, a measurement was taken every 30 minutes. | The absorbance was measured in a plate reader during 18h, a measurement was taken every 30 minutes. | ||
Sensing
Measuring GFP expression
-Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter.
This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc.
The absorbance was measured in a plate reader during 18h, a measurement was taken every 30 minutes.
"
