Team:TU Darmstadt/materials/Phusion

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Revision as of 17:37, 3 October 2013

Labbook | Protocols | Materials |

Contents

Phusion

About

Phusion is a Pyrococcus Pfu-like DNA polymerase with a dobule-strand DNA binding domain, resulting in an enhanced processivity and higher activity than other available DNA polymerases like taq and creates blunt ends.

Working conditions

  • 98°C denaturation temperature
  • ~30 s/kb extension velocity (at 72°C)
  • (TM-3)°C=TA

References

  • http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf
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