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Team:Paris Bettencourt/Notebook/Trojan Horse/Saturday 24th August.html
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Revision as of 17:57, 3 October 2013
Trojan Horse
ASDF24th August
Results: failure: as much GFP+ colonies growing on Kan than on LB
PCR from mini Gene sRNA anti Cm
35 H2O
10 Buffer 5x
1 dNTP
2 FW iT009
2 RV iT0010
0.2 minigene-plasmid amp from IDT
0.5 phusion
PCR Phusion Gradient around 53 °C
Time : 30 secondes
