Team:TU Darmstadt/protocols/Cell counting/plating

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Cell counting/plating

Materials
 LB agar plate
LB media
tubes

Procedure

  • Fill each tube in the dilution with 90 μl of LB
  • Add 10 μl of the sample to the first tube and mix
  • From the first tube, remove 10 μl and mix into second tube
  • Repeat for the number of dilutions you wish to do (8 should be more than enough)[2]
  • Take 10 μl from each dilution and spot it on to the agar plate
  • Allow droplet to dry and incubate

References
http://openwetware.org/wiki/Bacterial_cell_culture

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