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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | <font size = "4">Cell Surface Display</font> | + | <font size = "4">Cell Surface Display</font> <BR> |
Our Primers arrived today. <BR> | Our Primers arrived today. <BR> | ||
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We did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | We did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | ||
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
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<font size = "4">General</font> | <font size = "4">General</font> | ||
PCR
We did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
