Team:TU Darmstadt/materials

From 2013.igem.org

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{| style="color:green;background:none;"
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<center>
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!align="center"|[[Team:TU_Darmstadt/labbook|<span style="color:white;font-size:160%;"> Labbook |</span>]]
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<!-- central main menu -->
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!align="center"|[[Team:TU_Darmstadt/protocols|<span style="color:white;font-size:160%;"> Protocols |</span> ]]
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!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]]
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|}
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This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
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<br>
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<br>
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Can't find what you seek? Try their excellent page.
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<!-- Taskbar -->
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== Strains ==
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<div id="taskbar">
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*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
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<a href="https://2013.igem.org/Team:TU_Darmstadt">
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*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
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<img alt="Home_ausgewählt" src="/wiki/images/e/ef/Darmstadt_green_Home.jpg" width="70" height="30"></a>
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*[http://ecoliwiki.net/colipedia/index.php/BL21(DE3) BL21DE3]
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== Media ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/problem">
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*[https://2013.igem.org/Team:TU_Darmstadt/materials/DYT DYT]
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<img alt="Problem" src="/wiki/images/6/66/Darmstadt_green_Problem.jpg" width="100" height="30"></a>
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*[https://2013.igem.org/Team:TU_Darmstadt/materials/LB LB]
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*[https://2013.igem.org/Team:TU_Darmstadt/materials/SOB SOB]
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*[https://2013.igem.org/Team:TU_Darmstadt/materials/SOC SOC]
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== Antibiotics ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/solution">
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Ampicillin Ampicillin]
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<img alt="solution" src="/wiki/images/8/87/Darmstadt_green_Solution.jpg" width="100" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Chloramphenicol Chloramphenicol]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Kanamycin Kanamycin]
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== Enzymes ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/result">
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=== Phosphatases ===
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<img alt="result" src="/wiki/images/2/2c/Darmstadt_green_Result.jpg" width="80" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Antarctic_Phosphatase Antarctic Phosphatase]
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=== DNA polymerases ===
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Phusion Phusion]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Taq_polymerase Taq Polymerase]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Q5 Q5 Polymerase]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/InFusion In-Fusion HD Cloning Plus]
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=== DNA ligases ===
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/T4_DNA_Ligase T4 DNA ligase]
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=== Restriction enzymes ===
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/NheI NheI]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/EcoRI EcoRI]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/PstI PstI]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/SpeI SpeI]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/XbaI XbaI]
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== Electrophoresis ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/safety">
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=== Agarose gel electrophoresis ===
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<img alt="safety" src="/wiki/images/7/7a/Darmstadt_green_Safety.jpg" width="90" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/TriTrack_LoadingDye TriTrack loading dye]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/DNA_ladder DNA ladders]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Nancy Nancy]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/TAE TAE]
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=== PAGE ===
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<a href="https://2013.igem.org/Team:TU_Darmstadt/team">
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Coomassie_stain Coomassie stain]
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<img alt="team" src="/wiki/images/a/a4/Darmstadt_green_Team.jpg" width="70" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Destain Destain]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/SDS-Page SDS-Page]
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== Buffers ==
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<br>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/PBS PBS]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/TE TE]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Tris Tris]
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== Induction ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/strategy">
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Arabinose Arabinose]
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<img alt="team" src="/wiki/images/a/a0/Darmstadt_green_Strategy.jpg" width="100" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/IPTG IPTG]
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== DNA sequences ==
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<a href="https://2013.igem.org/Team:TU_Darmstadt/humanpractice">
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Plasmids Plasmids]
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<img alt="team" src="/wiki/images/4/4f/Darmstadt_green_Human_Practice.jpg" width="150" height="30"></a>
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Sequences Sequences]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/Primer Primer]
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<a href="https://2013.igem.org/Team:TU_Darmstadt/modelling">
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<img alt="team" src="/wiki/images/0/06/Darmstadt_green_Modelling.jpg" width="110" height="30"></a>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook">
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<img alt="team" src="/wiki/images/f/f3/Darmstadt_green_Labbook.jpg" width="90" height="30"></a>
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<h2><font size="6" color="#F0F8FF" face="Arial regular">Materials</font></h2>
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<font size="3" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions.
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<ul style="margin-left:50px; margin-right:50px; text-align:justify; ">
 +
<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/materials/Strains            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Strains    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Media            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Media    </b></font></a></li>
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/materials/Enzymes    "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Enzymes    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Buffers            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Buffers    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Stock_solutions        "><font size="3" color="#F0F8FF" face="Arial regular"><b> Stock solutions </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Chemicals            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Chemicals    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Primers            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Primers    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/gBlocks          "><font size="3" color="#F0F8FF" face="Arial regular"><b>  gBlocks  </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Plasmids           "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Plasmids   </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Consumabels            "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Consumabels    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/materials/Equipment          "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Equipment  </b></font></a></li>
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Revision as of 22:19, 3 October 2013




Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions.