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Team:Tuebingen/Notebook/Protocols/pcr
From 2013.igem.org
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Mix all reagents in the given order (Important: add polymerases last!) and transfer in one 0.2 mL Eppendorf-tube ("PCR-tube")</p> | + | <p>Mix all reagents in the given order (Important: add polymerases last!) and transfer in one 0.2 mL Eppendorf-tube ("PCR-tube"). Run cycler according to your requirements.</p> |
<p><b>This protocol yields exactly one single reaction mixture!</b> Scale linearly for multiple reaction mixtures and keep mixture on ice or even better at -20 °C for best results.</p> | <p><b>This protocol yields exactly one single reaction mixture!</b> Scale linearly for multiple reaction mixtures and keep mixture on ice or even better at -20 °C for best results.</p> | ||
Revision as of 00:52, 4 October 2013
PCR
Back to Protocols
Reagents
| 39.5 µL | Aqua dest. |
| 2.5 µL | dNTPs (200 µM each) |
| 1 µL | DNA (c = 100 ng/µL) |
| 5.0 µL | Taq-Buffer |
| 0.5 µL | Forward primer |
| 0.5 µL | Reverse primer |
| 1 µL | Taq/Pfu polymerases (9+1) |
Procedure
Mix all reagents in the given order (Important: add polymerases last!) and transfer in one 0.2 mL Eppendorf-tube ("PCR-tube"). Run cycler according to your requirements.
This protocol yields exactly one single reaction mixture! Scale linearly for multiple reaction mixtures and keep mixture on ice or even better at -20 °C for best results.
