"
Header
Up until now we succeded
• Producing Nanoparticles and loading them
• Clone a pH sensitive promoter
• Engineer a fusion protein between ice nucleation protein and
But we weren’t able to characterize and assemble the parts completely.
Contents |
What we would have done with more time:
Nanoparticles
•
•
•
Cell surface display of Streptavidin
• Cloned a plasmid that encodes a fusion protein INP-Streptavidin-YFP and one INP-YFP-Streptavidin. Then we would have been able to use the exact same protocol we used to characterize the Biobrick BBa_K523013, to proof surface localization.
•
•
Sensing/Effector
•
•
•
Taxi.Coli
• Cloned one of our Biobricks in a backbone that contains another resistance than chloramphenicol to cotransfect bacteria with the Plasmids responsible for Sensing/Effector and the Plasmid used to connect the Nanoparticles.
•
•
