Team:DTU-Denmark/Notebook/8 September 2013

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===Colony PCR to verify Nir in pZA21::ara-tight===
===Colony PCR to verify Nir in pZA21::ara-tight===
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Colony PCR on the Nir transformants, testing for NirH (primers 45a, 45b, annealing 59C), NirM+S (primers 44, 11a annealing 64C) and NirN (primers 43, 11b annealing 68C).
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program:
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{| class="wikitable" style="text-align: right"
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! temperature !! time !! cycles
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|-
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|  98C || 10:00 || -
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|-
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|  98C || 0:10 || 36
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|-
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|  59C/64C/68C || 0:30 || 36
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|-
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|  72C || 2:00 || 36
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|-
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|  72C || 5:00 || -
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|-
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|  10C || hold || -
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|-
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|}
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Colony PCR to verify HAO insert.
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Primers: 28a, 28b
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temperature: 59C
==Results==
==Results==

Revision as of 12:12, 4 October 2013

8 September

Contents

lab 208


Who was in the lab

Henrike, Kristian, Julia

Procedure

Colony PCR to verify Nir in pZA21::ara-tight

Colony PCR on the Nir transformants, testing for NirH (primers 45a, 45b, annealing 59C), NirM+S (primers 44, 11a annealing 64C) and NirN (primers 43, 11b annealing 68C).

program:

temperature time cycles
98C 10:00 -
98C 0:10 36
59C/64C/68C 0:30 36
72C 2:00 36
72C 5:00 -
10C hold -

Colony PCR to verify HAO insert.

Primers: 28a, 28b

temperature: 59C

Results


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