Team:DTU-Denmark/Bioreactor

From 2013.igem.org

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Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.
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Nitrous oxide concentrations measured in the bioreactor at the beginning (t=14:00) and end (t=22:00) of the experiment.
{| class="wikitable"
{| class="wikitable"
!Time
!Time
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!Replicate 1
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![N<sub>2</sub>O] Replicate 1
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!Replicate 2
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![N<sub>2</sub>O] Replicate 2
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!Replicate 3
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![N<sub>2</sub>O] Replicate 3
!Control: Distilled Water
!Control: Distilled Water
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Revision as of 13:50, 4 October 2013

Bioreactor

Contents


Description

The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anaerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.

The experiment was performed on September 17th.

Methods

Protocol Bioreactor

This image shows the bioreactor set up to run in triplicate.

Results

Nitrous oxide concentrations measured in the bioreactor at the beginning (t=14:00) and end (t=22:00) of the experiment.

Time [N2O] Replicate 1 [N2O] Replicate 2 [N2O] Replicate 3 Control: Distilled Water
14:00 1.84 mg/L 1.64 mg/L 1.60 mg/L N/A
22:00 1.43 mg/L 1.44 mg/L 1.50 mg/L 1.65 mg/L

Discussion

There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.