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Team:DTU-Denmark/Notebook/29 June 2013
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=208 lab= | =208 lab= | ||
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== Main purposes today == | == Main purposes today == | ||
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New PCR with x7-polymerase. | New PCR with x7-polymerase. | ||
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==who were in the lab== | ==who were in the lab== | ||
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Kristian | Kristian | ||
==Procedure== | ==Procedure== | ||
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Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | ||
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Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. | Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. | ||
Last program was 69°C annealing and 1:00 extension time with tube: 9+10. | Last program was 69°C annealing and 1:00 extension time with tube: 9+10. | ||
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==Results== | ==Results== | ||
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None of the PCRs worked. | None of the PCRs worked. | ||
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== Conclusion from today == | == Conclusion from today == | ||
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... | ... | ||
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Revision as of 08:09, 12 July 2013
29 June 2013
Contents |
208 lab
Main purposes today
New PCR with x7-polymerase.
who were in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates.
In tube 1+2, 5+6 where pZA21. In 9+10 GFP SF TAT In 3+4, 7+8 RFP
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.
Results
None of the PCRs worked.
Conclusion from today
...
