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Team:DTU-Denmark/Notebook/1 July 2013
From 2013.igem.org
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== Main purpose today == | == Main purpose today == | ||
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Make M9 Medium for the biolector toxicity experiment | Make M9 Medium for the biolector toxicity experiment | ||
==Who was in the lab== | ==Who was in the lab== | ||
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Helen, Gosia, Ariadni | Helen, Gosia, Ariadni | ||
==Procedure== | ==Procedure== | ||
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According to open wetware protocol for Biolector Experiment | According to open wetware protocol for Biolector Experiment | ||
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* 25 g NaCl | * 25 g NaCl | ||
*5 g NH4Cl | *5 g NH4Cl | ||
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| + | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 08:17, 12 July 2013
01 July 2013
Contents |
Main purpose today
Make M9 Medium for the biolector toxicity experiment
Who was in the lab
Helen, Gosia, Ariadni
Procedure
According to open wetware protocol for Biolector Experiment
1L of Media:
- 10 mL Casamino Acid ( ready solution from Andreas)
- 2 mM MgSO4 (from 2M liquid stock)
- 0.1 mM CaCl2 (liquid solution with 15% gly)
- 200 mL M9 salts
- Fill to 1 L with sterilized water
1 L stock of M9 salts:
- 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
- 15 g KH2PO4
- 25 g NaCl
- 5 g NH4Cl
