"
Team:TU Darmstadt/protocols/Glycerine stock
From 2013.igem.org
(Difference between revisions)
Kai15 (Talk | contribs)
(Created page with "<html> <style type="text/css"> body { margin:0; padding:15px 0 0; background-color: #ececec; background-image:url("https://static.igem.org/mediawiki/2013/1/14/Background_(3000x180_Pi...")
Newer edit →
(Created page with "<html> <style type="text/css"> body { margin:0; padding:15px 0 0; background-color: #ececec; background-image:url("https://static.igem.org/mediawiki/2013/1/14/Background_(3000x180_Pi...")
Newer edit →
Revision as of 17:29, 4 October 2013
Glycerine stock
Short explanation
In order to have a permanent culture of cells glycerine stocks can be made.
Procedure
1. Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
2. Freeze the stock at -20 °C
