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Inverted Microscopy
- All our constructs (ISA,ISD,ISI and competent cells as negative control) were incubated 1hr with TexRed conjugate nanoparticles.
- Visualization under the microscope with a cy5 filter.

Sensing-Effector

Innoculation of Bacteria with our constructs
-In order to characterize both the sensing and the effector plasmid I inoculated Bacteria in liquid LB cultures over-night.

Nanoparticles

Biotinylation
-We biotinylated the two new nanoparticles batches.
-End of the anti-strepta resistance check (sample mix, use of strepatavidin beads and microscopy)

DSL Measurements
-We did DLS measurements of the new biotinylated nanoparticles (before dialysis).