Team:EPF Lausanne/Calendar/30 September 2013

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<font size = "4"> Cell Surface Display</font> <BR>
<font size = "4"> Cell Surface Display</font> <BR>
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''Functionnal Assays'' <BR>
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''Functional Assays'' <BR>
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- Preparation of the samples incolulated the day before for immunostaining and biotin staining.
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- Preparation of the samples inoculated the day before for immunostaining and biotin staining.
<br>- Samples visualization with and inverted microscope using FITC filter.
<br>- Samples visualization with and inverted microscope using FITC filter.
<br>- The cells images and pattern were better for the biotin assay. We had nothing with the antibody.
<br>- The cells images and pattern were better for the biotin assay. We had nothing with the antibody.
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''OD measurements'' <BR>
''OD measurements'' <BR>
-I let the bacteria with the three promoters hya, cad and constitutive grow in media with different pHs. Each 45-70 minutes I measured the OD in order to see if the absence of GFP expression in the plate reader was due to non- induction of the promoter or the simple fact that the bacteria were dying at ceratin pH values.  
-I let the bacteria with the three promoters hya, cad and constitutive grow in media with different pHs. Each 45-70 minutes I measured the OD in order to see if the absence of GFP expression in the plate reader was due to non- induction of the promoter or the simple fact that the bacteria were dying at ceratin pH values.  
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Indeed this experiments showed, that the bacteria in the acidic milieu died very quickly which could explaind why the promoter was never induced.
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Indeed this experiments showed, that the bacteria in the acidic milieu died very quickly which could explained why the promoter was never induced.
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Latest revision as of 22:29, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
Functional Assays
- Preparation of the samples inoculated the day before for immunostaining and biotin staining.
- Samples visualization with and inverted microscope using FITC filter.
- The cells images and pattern were better for the biotin assay. We had nothing with the antibody.

Sensing-Effector

OD measurements
-I let the bacteria with the three promoters hya, cad and constitutive grow in media with different pHs. Each 45-70 minutes I measured the OD in order to see if the absence of GFP expression in the plate reader was due to non- induction of the promoter or the simple fact that the bacteria were dying at ceratin pH values. Indeed this experiments showed, that the bacteria in the acidic milieu died very quickly which could explained why the promoter was never induced.


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