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Team:TU Darmstadt/materials/Stock solutions
From 2013.igem.org
(Difference between revisions)
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3. use 1µL per 1mL medium<br><br> | 3. use 1µL per 1mL medium<br><br> | ||
</ul> | </ul> | ||
| - | < | + | <font size="4" color="#F0F8FF" face="Arial regular"> |
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | Kanamycin | ||
| + | </p></font> | ||
<ul> | <ul> | ||
1.Mix<br> | 1.Mix<br> | ||
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</ul> | </ul> | ||
2.sterile filtration<br> | 2.sterile filtration<br> | ||
| - | 3.aliquot in 1 mL stocks and store at -20°C | + | 3.aliquot in 1 mL stocks and store at -20°C<br> |
| + | 3. use 1µL per 1mL medium<br><br> | ||
</ul> | </ul> | ||
<br> | <br> | ||
Revision as of 03:47, 5 October 2013
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Stock solutions
Antibiotics
Ampicillin
Chloramphenicol
Kanamycin
1.Mix
2.sterile filtration
3. aliquot in 1 mL stocks and store at -20°C
4. use 1µL per 1mL medium
1.Mix
2.aliquot in 1mL stocks and store at -20°C
3. use 1µL per 1mL medium
1.Mix
2.sterile filtration
3.aliquot in 1 mL stocks and store at -20°C
3. use 1µL per 1mL medium
IPTG Isopropyl-beta-D-thiogalactopyranoside
1. Dissolve 238 mg IPTG in 10 mL DW
2. store in 1mL aliquots at -20°C
tritrack loading dye
