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Team:UC Chile
From 2013.igem.org
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<li class="pos1 sub_item oculto">BioBricks<a href="https://2013.igem.org/Team:UC_Chile/BioBricks"></a></li> | <li class="pos1 sub_item oculto">BioBricks<a href="https://2013.igem.org/Team:UC_Chile/BioBricks"></a></li> | ||
| - | <li class=" | + | <li class="pos2 sub_item oculto">Mathematical Model<a href="https://2013.igem.org/Team:UC_Chile/Mathematical Model"></a></li> |
| - | <li class=" | + | <li class="pos3 sub_item oculto">Wet Lab<a href="https://2013.igem.org/Team:UC_Chile/Wet Lab"></a></li> |
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<ul class="sub oculto"> | <ul class="sub oculto"> | ||
<li class="pos1 sub_item oculto">Home<a href="https://2013.igem.org/Team:UC_Chile/Human Practices"></a></li> | <li class="pos1 sub_item oculto">Home<a href="https://2013.igem.org/Team:UC_Chile/Human Practices"></a></li> | ||
| - | <li class=" | + | <li class="pos2 sub_item oculto">Synbio web<a href="https://2013.igem.org/Team:UC_Chile/Synbio web"></a></li> |
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| - | <div class="text"> | + | <div class="text">More</div> |
| - | <ul class="sub "> | + | <ul class="sub oculto"> |
| - | <li class="pos1 sub_item "> | + | <li class="pos1 sub_item oculto">Acknowledgments<a href="https://2013.igem.org/Team:UC_Chile/Acknowledgments"></a></li> |
| - | <li class=" | + | <li class="pos2 sub_item oculto">Biosafety<a href="https://2013.igem.org/Team:UC_Chile/Biosafety"></a></li> |
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<li class="pos3 sub_item oculto">Targeting<a href="https://2013.igem.org/Team:UC_Chile/Targeting"></a></li> | <li class="pos3 sub_item oculto">Targeting<a href="https://2013.igem.org/Team:UC_Chile/Targeting"></a></li> | ||
<li class="pos4 sub_item oculto">Purification<a href="https://2013.igem.org/Team:UC_Chile/Purification"></a></li> | <li class="pos4 sub_item oculto">Purification<a href="https://2013.igem.org/Team:UC_Chile/Purification"></a></li> | ||
| - | <li class=" | + | <li class="pos5 sub_item oculto">Disruption<a href="https://2013.igem.org/Team:UC_Chile/Disruption"></a></li> |
| - | <li class=" | + | <li class="pos5 sub_item oculto">In vitro Channeling<a href="https://2013.igem.org/Team:UC_Chile/In vitro Channeling"></a></li> |
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<div class="text">Methodology</div> | <div class="text">Methodology</div> | ||
<ul class="sub oculto"> | <ul class="sub oculto"> | ||
| - | <li class=" | + | <li class="pos1 sub_item oculto">Lab Notebook<a href="https://2013.igem.org/Team:UC_Chile/Lab NoteBook"></a></li> |
| - | <li class=" | + | <li class="pos2 sub_item oculto">Protocols<a href="https://2013.igem.org/Team:UC_Chile/Protocols"></a></li> |
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| - | + | <li class="pos1 sub_item oculto">Overview<a href="https://2013.igem.org/Team:UC_Chile/Overview"></a></li> | |
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| + | <h1>Overview</h1> | ||
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| + | Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production. | ||
| + | To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want: | ||
| + | A Whateversisome! | ||
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| + | We have successfully: | ||
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| + | <li>Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.</li> | ||
| + | <li>Found a targeting sequence to the inside of Carboxysome.</li> | ||
| + | <li>Designed an innovative methodology for purification and isolation of BMC.</li> | ||
| + | <li>We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.</li> | ||
| + | </ul><br> | ||
| + | Even more… | ||
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| + | <li> We are working towards generating step-by-step in vitro reactions.</li> | ||
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Latest revision as of 23:59, 7 October 2013
Overview
Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production.
To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want:
A Whateversisome!
- Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.
- Found a targeting sequence to the inside of Carboxysome.
- Designed an innovative methodology for purification and isolation of BMC.
- We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.
Even more…
- We are working towards generating step-by-step in vitro reactions.
