Team:NCTU Formosa/biobricks

(Difference between revisions)

Revision as of 13:21, 18 October 2013

Biobricks

To strive for the best results, we made a number of experiment. Our team always hang together in spite of many losses.

Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark.

The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).

This part, BBa_K1017401, includes our artificial sRNA-1 and rRBS-1. The non-coding small RNA can bind to the Shine-Dalgarno sequence on rRBS-1 by base-pairing. Once the rRBS-1 is blocked, ribosomes cannot bind to it to translate, thus, gene expressions downstream are decreased. Because of specific binding, rRBS-1 can only be bound by sRNA-1. We add P_lux upstream, so this part can be regulated by luxR/AHL complex.

This part, BBa_K1017402, is similar to BBa_K1017401 mentioned above, but without P_lux. rRBS-2 can only be bound by sRNA-2 due to specificity, then gene expressions downstream are decreased.

<groupparts>iGEM013 NCTU_Formosa</groupparts>

@@ Line 27: / Line 27: @@
 ====Temperature-regulated system====
+=====BBa_K1017602=====
 *[[File:Nctu_37rbs_mGFP.jpg|200px]]
+=====BBa_K1017603=====
 *[[File:Nctu_37rbs_luxr.jpg|200px]]
 </div></div>
 <div class="li"><div class="card">
 ====Small RNA-regulated system====
 *The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).