"
Team:NCTU Formosa/biobricks
From 2013.igem.org
(Difference between revisions)
Huierh04102 (Talk | contribs) (→BBa_K1017726) |
Huierh04102 (Talk | contribs) (→BBa_K1017726) |
||
| Line 8: | Line 8: | ||
====Light-regulated system==== | ====Light-regulated system==== | ||
======BBa_K1017726====== | ======BBa_K1017726====== | ||
| - | *pcya and ho1 are the enzymes needed to convert heme into PCB, the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use P<sub>cons<sub> upstream in order to make E.coli continuously expressed them to synthesize PCB. | + | *pcya and ho1 are the enzymes needed to convert heme into PCB, the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use P<sub>cons</sub> upstream in order to make E.coli continuously expressed them to synthesize PCB. |
<br>[[File:Nctu_pcya_ho1.jpg|450px]] | <br>[[File:Nctu_pcya_ho1.jpg|450px]] | ||
Revision as of 10:30, 20 October 2013
Biobricks
To strive for the best results, we made a number of experiment. Our team always hang together in spite of many losses.
Contents |
Parts submitted to the Registry
Light-regulated system
BBa_K1017726
- pcya and ho1 are the enzymes needed to convert heme into PCB, the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use Pcons upstream in order to make E.coli continuously expressed them to synthesize PCB.
BBa_K1017301
BBa_K1017781
BBa_K1017101
- Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark.
Temperature-regulated system
BBa_K1017602
- By using mGFP as a reporter gene, we can test whether the 37 °C RBS works.
BBa_K1017603
- In our circuit, this biobrick is the part of Plux's activation when the temperature reaches to 37oC.
Small RNA-regulated system
BBa_K1017403
BBa_K1017404
BBa_K1017202
- The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).
BBa_K1017811
BBa_K1017401
- This part, BBa_K1017401, includes our artificial sRNA-1 and rRBS-1. The non-coding small RNA can bind to the Shine-Dalgarno sequence on rRBS-1 by base-pairing. Once the rRBS-1 is blocked, ribosomes cannot bind to it to translate, thus, gene expressions downstream are decreased. Because of specific binding, rRBS-1 can only be bound by sRNA-1. We add Plux upstream, so this part can be regulated by luxR/AHL complex.
BBa_K1017402
- This part, BBa_K1017402, is similar to BBa_K1017401 mentioned above, but without Plux. rRBS-2 can only be bound by sRNA-2 due to specificity, then gene expressions downstream are decreased.
<groupparts>iGEM013 NCTU_Formosa</groupparts>
