Team:HokkaidoU Japan/Shuffling Kit/Future Work
From 2013.igem.org
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<h2>To make Promoter Selector better kit</h2> | <h2>To make Promoter Selector better kit</h2> | ||
- | <p>To make Promoter Selector better, we are going to | + | <p>To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove pigment producing constructs by digesting with PstI.</p> |
<div class="fig fig800"> | <div class="fig fig800"> | ||
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<p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in case of 2 CDS, 64 operons in case of 3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p> | <p>We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in case of 2 CDS, 64 operons in case of 3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.</p> | ||
- | <p>For example PHB | + | <p> |
- | (polyhydroxybutyrate) ,3 enzymes synthesize PHB from substrate.PHB is one of biodegradable plastic. You can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. | + | In the case of producing valuable molecules inside the cell, it is important to select the RBS which results the largest yield. |
- | + | ||
+ | |||
+ | For example PHB | ||
+ | (polyhydroxybutyrate) ,3 enzymes synthesize PHB from substrate. PHB is one of the eco-friendly, biodegradable plastic. You can learn more about PHB at <a href="https://2012.igem.org/Team:HokkaidoU_Japan/Project/PHB_Synthesis">iGEM HokkaidoU_Japan 2012 wiki</a>. In the production of PHB, each CDS has the optimum RBS. Our RS can be used for selecting the best combination of RBSs. | ||
+ | |||
+ | |||
+ | |||
+ | It is possible to use RBS Selector when producing medicine or vaccine etc. | ||
+ | |||
</p> | </p> | ||
<div class="fig fig800"> | <div class="fig fig800"> |
Revision as of 19:35, 28 October 2013
Maestro E.coli
Optimization Kit
![](https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png)
Maestro E.coli Future Work
To make Promoter Selector better kit
To make Promoter Selector better, we are going to place pigment producing construct between two PstI sites. After you confirm the best promoter, you can remove pigment producing constructs by digesting with PstI.
![](https://static.igem.org/mediawiki/2013/1/12/Fig11_new2_800_HokkaidoU_2013.png)
Application of RBS Selector
We can get a lot of operons by RBS Selector. RBS Selector provides 16 operons in case of 2 CDS, 64 operons in case of 3 CDS. What can we do using these number of operons? We expect industrial applications of RBS Selector as future works.
In the case of producing valuable molecules inside the cell, it is important to select the RBS which results the largest yield. For example PHB (polyhydroxybutyrate) ,3 enzymes synthesize PHB from substrate. PHB is one of the eco-friendly, biodegradable plastic. You can learn more about PHB at iGEM HokkaidoU_Japan 2012 wiki. In the production of PHB, each CDS has the optimum RBS. Our RS can be used for selecting the best combination of RBSs. It is possible to use RBS Selector when producing medicine or vaccine etc.
![](https://static.igem.org/mediawiki/2012/3/38/HokkaidoU_PHB_Fig3.jpg)
Select Promoter & RBS
To select proteins expressions in wider range, you can use Promoter Selector and RBS Selector at the same time! The combination number is 320 patterns!
![](https://static.igem.org/mediawiki/2013/3/3b/Maestro_HokkaidoU_2013.png)
Besides, you can create promoter and terminator by yourself and assemble them with our RBS Selector and GGA VECTOR (K1084301) by Golden Gate Assembly!