BioBrick
Favorite Tianjin 2013 iGEM Team Parts
| Name |
Type |
Description |
Designer |
Length |
| Bba_K1020005 |
Composite |
Alkane Marker--Device for detection of various alkanes |
Boxuan Zeng
Yue Liu |
2146bp |
| Bba_K1020012 |
Composite |
Alkane Selector for intracellular alkane production |
Boxuan Zeng
Yue Liu |
2662bp |
Tianjin 2013 iGEM Team Parts Sandbox
| Name |
Type |
Description |
Designer |
Length |
| Bba_K1020001 |
Reporter |
PalkM promoter with reporter gene |
Boxuan Zeng
Yue Liu |
1013bp |
| Bba_K1020002 |
Coding |
alkane regulatory protein ALKR |
Boxuan Zeng |
927bp |
| Bba_K1020003 |
Reporter |
AlkR -PSB1C3-Low constitutive |
Yue Liu |
1125bp |
| Bba_K1020004 |
Composite |
Biosensor for alkane detection |
Yue Liu
Su Wang |
2009bp |
| Bba_K1020006 |
Coding |
YbhGFSR |
Boxuan Zeng
Yang Wu |
4971bp |
| Bba_K1020007 |
Composite |
AlkR -PSB1C3-High constitutive |
Yue Liu |
1125bp |
| Bba_K1020008 |
Composite |
alkane producing module |
Boxuan Zeng
Yue Liu |
1809bp |
| Bba_K1020009 |
Composite |
YbhGFSR operon |
Boxuan Zeng |
5034bp |
| Bba_K1020010 |
Coding |
TetA gene under the control of PalkM |
Boxuan Zeng
Shoujie Sun |
1529bp |
| Bba_K1020011 |
Composite |
High expressed Alkane Selector for intracellular alkane production |
Boxuan Zeng
Qingyi Deng |
2662bp |
Others
Results for Agarose Gel Electrophoresis:
Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT
Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT
Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT
Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3
Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3
Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3
[[Image:TJU-PROJECT-C3.jpg|thumb|600px|center|
Figure 3. The visible color change of the strain cultured in 3mL M9 medium with different alkanes induced for 12 hours.]]
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