Team:BostonU/Protocols

From 2013.igem.org

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<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>

Revision as of 14:37, 19 July 2013



Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.


  • DNA Quantification

  • Gel Extraction

  • Miniprep

  • PCR Cleanup

  • Ligation

  • Media Prep

  • MoClo Level 0 Reaction

  • MoClo Level 1 and 2 Reactions

  • Phusion PCR

  • Restriction Digest

  • Restriction Digest 10 µL mapping

  • Transformation

    Retrieved from "http://2013.igem.org/Team:BostonU/Protocols"