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Revision as of 11:20, 20 July 2013
Enter the nucleotide sequence below!
Enter sequence!
Sample Output
| Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator] | |||||||||||||||||||
| BioBrick: BBa_K1159000 | Used open reading frame from position ?x? to ?y?. | ||||||||||||||||||
| A (Ala) | 1 (5.00%) | R (Arg) | 1 (5.00%) | N (Asn) | 1 (5.00%) | D (Asp) | 2 (10.00%) | C (Cys) | 1 (5.00%) | ||||||||||
| Q (Gln) | 1 (5.00%) | E (Glu) | 2 (10.00%) | G (Gly) | 1 (5.00%) | H (His) | 1 (5.00%) | I (Ile) | 0 (0.00%) | ||||||||||
| L (Leu) | 0 (0.00%) | K (Lys): | 1 (5.00%) | M (Met) | 1 (5.00%) | F (Phe) | 2 (10.00%) | P (Pro) | 1 (5.00%) | ||||||||||
| S (Ser) | 1 (5.00%) | T (Thr) | 0 (0.00%) | W (Trp) | 1 (5.00%) | Y (Tyr) | 1 (5.00%) | V (Val) | 1 (5.00%) | ||||||||||
| Amino acid counting: | Total number of amino acids (aa): | 20 | Number of positively charged aa (Arg + Lys): | 2 | Number of negatively charged aa (Asp + Glu): | 4 | |||||||||||||
| Biochemical parameters: | Molecular weight [Da]: | 2459.69 | Theoretical pI: | 4.75 | Extinction coefficient: | 7052.5 (all Cys as cystine), 6990 (no Cys as cystine) | |||||||||||||
| Estimated half-life: | Mammals: | 4.4 hour | Yeast: | >20 hour | E. coli: | >10 hour | |||||||||||||
| Codon usage: | Mammals: | ?good? | Yeast: | ?Ok? | E. coli: | ?bad ? | |||||||||||||
| RFC standard: | This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends. | ||||||||||||||||||
| The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description. | |||||||||||||||||||
