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Team:BostonU/PUP
From 2013.igem.org
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| + | <h5>June 5, 2013</h5> | ||
| + | Today was our first Puppeteer meeting so we were able to meet the computational (comp) part of the CIDAR lab that we'll be working with for the project. The people working on this project include Traci, Shawn, Pooja, Devina, Swapnil, Janoo, Alejandro, Denise, and Eda. We also got a better idea of what Puppeteer can do and the capabilities of the liquid handling robot (TECAN). The team discussed goals for the summer as well as the differences between a human running an experiment and a robot running the same one. | ||
| - | + | <h5>June 11,2013</h5> | |
| - | + | This meeting we talked about what exactly we wanted to accomplished this summer in greater detail. We reached an agreement that the robot will perform 10 level 1 MoClo reactions that will all have J23100_EB, E0030_CD, B0015_DF, and DVL1_EF. Only the RBS will change in each construct. We will be using B0032_BC, B0034_BC, B0034m1_BC, B00343m2_BC, BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, and BCD16_BC. | |
| + | The wet lab team then showed the comp team the various protocols that would be required to make these 10 devices. <img src=”https://static.igem.org/mediawiki/2013/a/a1/PUP_061113.jpg”/> The comp team then discussed designing a protocol map. | ||
| + | Since there will be many experiments using the above parts, the wet lab team decided to prepare extra plasmid stocks. | ||
Revision as of 14:47, 22 July 2013
Puppeteer Notebook
June 5, 2013
Today was our first Puppeteer meeting so we were able to meet the computational (comp) part of the CIDAR lab that we'll be working with for the project. The people working on this project include Traci, Shawn, Pooja, Devina, Swapnil, Janoo, Alejandro, Denise, and Eda. We also got a better idea of what Puppeteer can do and the capabilities of the liquid handling robot (TECAN). The team discussed goals for the summer as well as the differences between a human running an experiment and a robot running the same one.June 11,2013
This meeting we talked about what exactly we wanted to accomplished this summer in greater detail. We reached an agreement that the robot will perform 10 level 1 MoClo reactions that will all have J23100_EB, E0030_CD, B0015_DF, and DVL1_EF. Only the RBS will change in each construct. We will be using B0032_BC, B0034_BC, B0034m1_BC, B00343m2_BC, BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, and BCD16_BC. The wet lab team then showed the comp team the various protocols that would be required to make these 10 devices.
The comp team then discussed designing a protocol map.
Since there will be many experiments using the above parts, the wet lab team decided to prepare extra plasmid stocks.
