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Team:BostonU/NotebookML
From 2013.igem.org
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<h2>May</h2> | <h2>May</h2> | ||
<h10>Week of May 12, 2013</h10> | <h10>Week of May 12, 2013</h10> | ||
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<ul> | <ul> | ||
<li>Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically <a href = “https://2013.igem.org/Team:BostonU/HK”>histidine kinase</a> and <a href=”https://2013.igem.org/Team:BostonU/QS”>quorum sensing</a>.</li> | <li>Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically <a href = “https://2013.igem.org/Team:BostonU/HK”>histidine kinase</a> and <a href=”https://2013.igem.org/Team:BostonU/QS”>quorum sensing</a>.</li> | ||
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<li>Stocked up on lab supplies (plates, broth, plasmids)</li> | <li>Stocked up on lab supplies (plates, broth, plasmids)</li> | ||
</ul> | </ul> | ||
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| + | <h10>Week of May 19, 2013</h10> | ||
| + | <ul> | ||
| + | <li>Began to create new bicistronic design RBS’s (BCDs): used PCR on BCD2 template to make BCD1, followed with LV0 reaction</li> | ||
| + | <li>Began to restock low concentration and low volume plasmid for level 0 parts</li> | ||
| + | </ul> | ||
| + | <br> | ||
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| + | <h10>Week of May 26, 2013</h10> | ||
| + | <ul> | ||
| + | <li>Sent BCD1 made last week for sequence verification</li> | ||
| + | <li>Assembled Lv1 circuits to test out new fluorescent proteins (CyPet, DsRed, mCitrine, mOrange) and for flow cytometry training</li> | ||
| + | <li>Ran flow cytometry with Traci for our training</li> | ||
| + | <li>Continued to restock low concentration and low volume parts</li> | ||
| + | </ul> | ||
| + | <br> | ||
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Revision as of 13:55, 23 July 2013
Characterization Notebook
- Assemble level 0 parts needed
- Constitutive Promoter Characterization Project:
- J23100_AB, J23113_AB, J23115_AB, J23115_EB, J23116_AB, J23116_EB, J23117_AB, J23118_AB, J23119_AB
- BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, BCD16_BC: completed 6/27/13
- Repressible Promoter Characterization Project:
- C0012 (LacI), C0062_CD (LuxR), C0071_CD (rhlR_CD), pLacI (R0010_FB)
- Assemble level 1 circuits for characterization of Constitutive Promoter Library
- 160 circuits that have different combinations of J231XX promoters and RBS’s
- 1 set with all promoters + BCD2
- 1 set with all RBS’s+J23100
- Assemble level 1 circuits for characterization of Repressible Promoter
- Run flow cytometry on the promoter characterization circuits
- Assemble level 2 inverter as an example of the library’s use
- Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: completed:6/21/13
May
- Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically histidine kinase and quorum sensing.
- Learned about Clotho software suite and learned how to use Eugene, RavenCAD, and Pigeon from other members of the CIDAR lab.
- Familiarized ourselves with protocols and lab etiquette
- Stocked up on lab supplies (plates, broth, plasmids)
- Began to create new bicistronic design RBS’s (BCDs): used PCR on BCD2 template to make BCD1, followed with LV0 reaction
- Began to restock low concentration and low volume plasmid for level 0 parts
- Sent BCD1 made last week for sequence verification
- Assembled Lv1 circuits to test out new fluorescent proteins (CyPet, DsRed, mCitrine, mOrange) and for flow cytometry training
- Ran flow cytometry with Traci for our training
- Continued to restock low concentration and low volume parts
