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Team:Groningen/Labwork/25 June 2013
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<br/>A couple of media are prepared: | <br/>A couple of media are prepared: | ||
<br/>LB medium with agar 1 liter | <br/>LB medium with agar 1 liter | ||
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'''Mirjam and Claudio''' | '''Mirjam and Claudio''' | ||
<br/>''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged. | <br/>''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged. | ||
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Revision as of 11:48, 24 July 2013
Mirjam, Chaline and Inne
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove. '''Mirjam and Claudio'''
''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.
