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29/07/13
From 2013.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | #Ran the gel from the 26/07/2013 again for 45min | |
| - | + | #Digestion of plasmid backbone (pSB1C3) | |
| + | *Three different reactions | ||
| + | *Master Mix (changes were made from the protocol) | ||
| + | **5ul NEB Buffer 2 | ||
| + | **0.5ul of EcoRI | ||
| + | **0.5ul of PstI | ||
| + | **19ul of dH2O | ||
| + | *For each reaction add: | ||
| + | **4ul of linearized backbone | ||
| + | **4ul of enzyme master mix | ||
| + | *digest at 37C for 30min | ||
| + | *heat kill at 80C for 20min | ||
Revision as of 13:38, 29 July 2013
- Ran the gel from the 26/07/2013 again for 45min
- Digestion of plasmid backbone (pSB1C3)
- Three different reactions
- Master Mix (changes were made from the protocol)
- 5ul NEB Buffer 2
- 0.5ul of EcoRI
- 0.5ul of PstI
- 19ul of dH2O
- For each reaction add:
- 4ul of linearized backbone
- 4ul of enzyme master mix
- digest at 37C for 30min
- heat kill at 80C for 20min
