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Team:Groningen/Labwork/3 July 2013
From 2013.igem.org
(Difference between revisions)
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<h2>Mirjam</h2> | <h2>Mirjam</h2> | ||
| - | <br/>Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes. | + | <br/>Run a 0.8% agarose <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> to examine the purified promoter, gel run at 100V for 22 minutes. |
<h2>Mirjam and Sander</h2> | <h2>Mirjam and Sander</h2> | ||
| - | <br/>The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. | + | <br/>The gel examined that again dimerization of the primers happened. Therefore a new <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> run is made with the addition of DMSO. |
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol). | Turned out that the one with DMSO had better results. Did a gel purification (standard protocol). | ||
<h2>Sander and Inne</h2> | <h2>Sander and Inne</h2> | ||
| - | <br/>Did a PCR for the silk template, in duplo. | + | <br/>Did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> for the silk template, in duplo. |
With the following protocol: | With the following protocol: | ||
<br>1 2 3 4 5 6 | <br>1 2 3 4 5 6 | ||
| Line 40: | Line 40: | ||
NB: another change is that we used 5% DMSO this time | NB: another change is that we used 5% DMSO this time | ||
| - | Ran a gel which had more bigger bands them the previous pcr. | + | Ran a <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> which had more bigger bands them the previous pcr. |
The samples without DMSO were clearer than those with DMSO. | The samples without DMSO were clearer than those with DMSO. | ||
Latest revision as of 09:45, 30 July 2013
Mirjam
Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.
Mirjam and Sander
The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).
Sander and Inne
Did a PCR for the silk template, in duplo. With the following protocol:
1 2 3 4 5 6
98 98 85 72 72 4
2:00 0:30 0:25 0:27 10:00 forever So two with DMSO and two without. NB: another change is that we used 5% DMSO this time Ran a gel which had more bigger bands them the previous pcr. The samples without DMSO were clearer than those with DMSO.
