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Team:UTK-Knoxville/4 June 2013
From 2013.igem.org
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| - | <h1><span class="mw-headline">June | + | <h1><span class="mw-headline">June 4th 2013</span></h1> |
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<li>The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58°C, 60°C, and 64°C. Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li> | <li>The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58°C, 60°C, and 64°C. Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li> | ||
Latest revision as of 20:00, 30 July 2013
June 4th 2013
- The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58°C, 60°C, and 64°C. Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.
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