Phage Purification

From 2013.igem.org

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Revision as of 21:25, 15 May 2013

March

3/15/13

Today we began researching for a procedure to begin purifying phage. We have found that T7 can self assemble with scaffolding proteins without forming procapsids, and that T4 has only been know to form procapsids. We may not have to worry about T4 if the other group isn't using it.

Important findings - Phage have been purified before Phage capsids can self assemble Phage (amber strain) can have their genes knocked out to make a hollow phage

3/18/13

Priorities List: Find out how to put drugs into the capsid Possibly contact F.W. Studier for his amber t7 phage strain Next class we plan on growing up phage so that we will have a decent amount of phage to work with. We have several procedures that we plan on testing so that we can see if we can purify the protein capsid. We hope to be proceeding with these as soon as we have phage that we can use. Another issue that we need to consider is how to get drugs into the capsid. We need to be able to test and see if we can actually fill our empty capsids with a material. We have several procedures found in other papers that could possibly help us with this.

3/20/13

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April

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June

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July

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September

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@@ Line 11: / Line 11: @@
 ===3/18/13===
+Priorities List:
+Find out how to put drugs into the capsid
+Possibly contact F.W. Studier for his amber t7 phage strain
+Next class we plan on growing up phage so that we will have a decent amount of phage to work with.  We have several procedures that we plan on testing so that we can see if we can purify the protein capsid.  We hope to be proceeding with these as soon as we have phage that we can use.  Another issue that we need to consider is how to get drugs into the capsid.  We need to be able to test and see if we can actually fill our empty capsids with a material.  We have several procedures found in other papers that could possibly help us with this.
 ===3/20/13===