06/08/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Running of an agarose gel== *Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.")
(→Running of an agarose gel)
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==Running of an agarose gel==
==Running of an agarose gel==
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
 +
 +
==Making chloroamphenicol agar plates==
 +
*Making additional plates
 +
**400ml of agar
 +
***Melt in microwave
 +
***3mins on low -> shake
 +
***Repeat
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***2mins on low -> shake
 +
***Repeat
 +
**Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
 +
**Pour out onto 20 petri dishes
 +
**Leave to set on bench

Revision as of 10:03, 6 August 2013

Running of an agarose gel

  • Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.

Making chloroamphenicol agar plates

  • Making additional plates
    • 400ml of agar
      • Melt in microwave
      • 3mins on low -> shake
      • Repeat
      • 2mins on low -> shake
      • Repeat
    • Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
    • Pour out onto 20 petri dishes
    • Leave to set on bench
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