Team:Evry/Amorces
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<a href='https://2013.igem.org/Team:Evry/Primers' title='To English page'> <img src='https://static.igem.org/mediawiki/2013/7/78/Anglais.gif'/></a> | <a href='https://2013.igem.org/Team:Evry/Primers' title='To English page'> <img src='https://static.igem.org/mediawiki/2013/7/78/Anglais.gif'/></a> | ||
+ | <h1 style="text-align:center"> Primers </h1> | ||
+ | |||
+ | <table width="100%" cellpadding="10" cellspacing="0" align='center' border="1"> | ||
+ | <tr> | ||
+ | <th align="center"> | ||
+ | <b>CODE</b> | ||
+ | </th> | ||
+ | <th class="colonne2" align="center" id="col2"> | ||
+ | <div style="width: 200px;"><b>NAME</b></div> | ||
+ | </th> | ||
+ | <th align="center"> | ||
+ | <b>LENGTH</b> | ||
+ | </th> | ||
+ | <th align="center"> | ||
+ | <b>ANNEALING<br/>TEMPERATURE</b> | ||
+ | </th> | ||
+ | <th align="center" width="200px"> | ||
+ | <div style="width:"200px";"<b>SEQUENCE </b></div> | ||
+ | </th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P001 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Primer Fur<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 41 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P002 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Primer Fur<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 48 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 77°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P005 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS B0034<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P006 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS B0034<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P007 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | aceB Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 42 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P008 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | ace B Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 32 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 78°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P009 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent C Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 49 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P010 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent C Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 32 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 64°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ACTAGTAATCATCCTCCACAAAATGATAAAGG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P011 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fec A Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 50 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P012 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fec A Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 41 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P013 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fec C Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 42 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 77°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P014 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fec C Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 36 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P015 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23100<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 84°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCT<br/>AGCGTGATGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P016 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23100<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 84°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAG<br/>GCTGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P017 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°1<br/>(Foward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 45 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P018 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°1<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 45 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P019 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°2<br/>(Foward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P020 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°2<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P021 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fep A Natural Fur promoter<br/>(Foward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 41 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P022 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fep A Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 42 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P023 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fes Natural Fur promoter<br/>(Foward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 40 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 77°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P024 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fes Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 37 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P025 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Sdh C Natural Fur promoter<br/>(Foward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 45 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P026 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Sdh C Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 36 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P027 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | ybi L Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 50 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P028 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | ybi L Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 50 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P029 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | ync E Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 40 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 78°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P030 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | ync E Natural Fur promoter<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 39 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P031 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°3<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P032 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°3<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P033 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°4<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P034 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°4<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P035 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°5<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P036 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°5<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P037 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°6<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P038 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°6<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P039 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°7<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 71°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P040 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°7<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 71°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P041 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°8<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P042 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°8<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P043 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°9<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P044 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°9<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P045 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°10<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P046 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°10<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P047 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°11<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P048 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°11<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P049 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°12<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P050 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°12<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P051 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°13<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P052 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°13<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P053 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°14<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P054 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°14<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P055 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°15<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P056 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Fur Binding Site N°15<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 73°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P057 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23101<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 81°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCT<br/>AGCGTGATGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P058 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23101<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 81°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAG<br/>GCTGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P059 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23102<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 83°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCT<br/>AGCGTGATGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P060 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23102<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 83°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAG<br/>GCTGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P061 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23104<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 82°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCT<br/>AGCGTGATGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P062 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Andersen's promoter J23104<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 82°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAG<br/>GCTGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P063 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | VR primer iGEM standard | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 20 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 63°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ATTACCGCCTTTGAGTGAGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P064 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | VF2 primer iGEM standard | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 20 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 64°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | TGCCACCTGACGTCTAAGAA | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P065 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent A Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 81°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATG<br/>GATTTCAGCGGTAAAAATG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P066 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent A Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 45 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 81°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P067 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent B Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 74 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 78°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGG<br/>CTATTCCAAAATTACAGGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P068 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent B Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 45 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 79°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P069 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent C Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 68 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 80°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGG<br/>ATACGTCACTGGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P070 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent C Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 53 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P071 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent D Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 70 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 83°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCG<br/>ATATGAAAACTACGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P072 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent D Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 79°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P073 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent E Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 71 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 80°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATG<br/>AGCATTCCATTCACCC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P074 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent E Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 43 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 81°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P075 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent F Biosynthesis of Enterobactin Siderophore<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 68 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCA<br/>GCATTTACCTTTG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P076 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Ent F Biosynthesis of Enterobactin Siderophore<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 47 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 78°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P077 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS sfGFP - Plasmid 3<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 65 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAA<br/>AGGCGAAGAG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P078 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS sfGFP - Plasmid 3<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 58 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 75°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P079 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Promoteur Lac + Lac Operator<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 93 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 82°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAG<br/>CGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P080 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Promoteur Lac + Lac Operator<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 93 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 82°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGT<br/>TATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P081 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS positive control - Plasmid 2<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 46 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 77°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P082 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | RBS positive control - Plasmid 2<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 46 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 77°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P083 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Lac I - Plasmid 1/2<br/>(Forward - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 40 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 72°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | P084 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | Lac I - Plasmid 1/2<br/>(Reverse - GoldenBrick sequence) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 56 nt | ||
+ | </p> | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | <p> | ||
+ | 76°C | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h1 align='center'>Amorces</h1> | <h1 align='center'>Amorces</h1> |
Revision as of 13:34, 6 August 2013
Primers
CODE |
NAME
|
LENGTH |
ANNEALING TEMPERATURE |
SEQUENCE
|
---|---|---|---|---|
P001 |
Primer Fur |
41 nt |
74°C |
CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC |
P002 |
Primer Fur |
48 nt |
77°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG |
P005 |
RBS B0034 |
47 nt |
75°C |
GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA |
P006 |
RBS B0034 |
47 nt |
75°C |
TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC |
P007 |
aceB Natural Fur promoter |
42 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC |
P008 |
ace B Natural Fur promoter |
32 nt |
78°C |
TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG |
P009 |
Ent C Natural Fur promoter |
49 nt |
73°C |
GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG |
P010 |
Ent C Natural Fur promoter |
32 nt |
64°C |
ACTAGTAATCATCCTCCACAAAATGATAAAGG |
P011 |
Fec A Natural Fur promoter |
50 nt |
73°C |
GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC |
P012 |
Fec A Natural Fur promoter |
41 nt |
74°C |
CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG |
P013 |
Fec C Natural Fur promoter |
42 nt |
77°C |
GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC |
P014 |
Fec C Natural Fur promoter |
36 nt |
75°C |
CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG |
P015 |
Andersen's promoter J23100 |
70 nt |
84°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCT |
P016 |
Andersen's promoter J23100 |
70 nt |
84°C |
CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAG |
P017 |
Fur Binding Site N°1 |
45 nt |
72°C |
CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC |
P018 |
Fur Binding Site N°1 |
45 nt |
72°C |
GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG |
P019 |
Fur Binding Site N°2 |
47 nt |
75°C |
CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC |
P020 |
Fur Binding Site N°2 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG |
P021 |
Fep A Natural Fur promoter |
41 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG |
P022 |
Fep A Natural Fur promoter |
42 nt |
73°C |
CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC |
P023 |
Fes Natural Fur promoter |
40 nt |
77°C |
GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG |
P024 |
Fes Natural Fur promoter |
37 nt |
75°C |
CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG |
P025 |
Sdh C Natural Fur promoter |
45 nt |
75°C |
GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC |
P026 |
Sdh C Natural Fur promoter |
36 nt |
76°C |
CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC |
P027 |
ybi L Natural Fur promoter |
50 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC |
P028 |
ybi L Natural Fur promoter |
50 nt |
72°C |
CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG |
P029 |
ync E Natural Fur promoter |
40 nt |
78°C |
GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG |
P030 |
ync E Natural Fur promoter |
39 nt |
75°C |
CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC |
P031 |
Fur Binding Site N°3 |
47 nt |
75°C |
CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC |
P032 |
Fur Binding Site N°3 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG |
P033 |
Fur Binding Site N°4 |
47 nt |
72°C |
CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC |
P034 |
Fur Binding Site N°4 |
47 nt |
72°C |
GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG |
P035 |
Fur Binding Site N°5 |
47 nt |
70°C |
CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC |
P036 |
Fur Binding Site N°5 |
47 nt |
70°C |
GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG |
P037 |
Fur Binding Site N°6 |
47 nt |
74°C |
CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC |
P038 |
Fur Binding Site N°6 |
47 nt |
74°C |
GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG |
P039 |
Fur Binding Site N°7 |
47 nt |
71°C |
CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC |
P040 |
Fur Binding Site N°7 |
47 nt |
71°C |
GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG |
P041 |
Fur Binding Site N°8 |
47 nt |
73°C |
CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC |
P042 |
Fur Binding Site N°8 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG |
P043 |
Fur Binding Site N°9 |
47 nt |
73°C |
CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC |
P044 |
Fur Binding Site N°9 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG |
P045 |
Fur Binding Site N°10 |
47 nt |
75°C |
CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC |
P046 |
Fur Binding Site N°10 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG |
P047 |
Fur Binding Site N°11 |
47 nt |
74°C |
CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC |
P048 |
Fur Binding Site N°11 |
47 nt |
74°C |
GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG |
P049 |
Fur Binding Site N°12 |
47 nt |
73°C |
CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC |
P050 |
Fur Binding Site N°12 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG |
P051 |
Fur Binding Site N°13 |
47 nt |
75°C |
CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC |
P052 |
Fur Binding Site N°13 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG |
P053 |
Fur Binding Site N°14 |
47 nt |
73°C |
CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC |
P054 |
Fur Binding Site N°14 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG |
P055 |
Fur Binding Site N°15 |
47 nt |
73°C |
CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC |
P056 |
Fur Binding Site N°15 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG |
P057 |
Andersen's promoter J23101 |
70 nt |
81°C |
GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCT |
P058 |
Andersen's promoter J23101 |
70 nt |
81°C |
CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAG |
P059 |
Andersen's promoter J23102 |
70 nt |
83°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCT |
P060 |
Andersen's promoter J23102 |
70 nt |
83°C |
CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAG |
P061 |
Andersen's promoter J23104 |
70 nt |
82°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCT |
P062 |
Andersen's promoter J23104 |
70 nt |
82°C |
CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAG |
P063 |
VR primer iGEM standard |
20 nt |
63°C |
ATTACCGCCTTTGAGTGAGC |
P064 |
VF2 primer iGEM standard |
20 nt |
64°C |
TGCCACCTGACGTCTAAGAA |
P065 |
Ent A Biosynthesis of Enterobactin Siderophore |
74 nt |
81°C |
GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATG |
P066 |
Ent A Biosynthesis of Enterobactin Siderophore |
45 nt |
81°C |
GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG |
P067 |
Ent B Biosynthesis of Enterobactin Siderophore |
74 nt |
78°C |
GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGG |
P068 |
Ent B Biosynthesis of Enterobactin Siderophore |
45 nt |
79°C |
GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG |
P069 |
Ent C Biosynthesis of Enterobactin Siderophore |
68 nt |
80°C |
GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGG |
P070 |
Ent C Biosynthesis of Enterobactin Siderophore |
53 nt |
76°C |
GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC |
P071 |
Ent D Biosynthesis of Enterobactin Siderophore |
70 nt |
83°C |
GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCG |
P072 |
Ent D Biosynthesis of Enterobactin Siderophore |
47 nt |
79°C |
GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC |
P073 |
Ent E Biosynthesis of Enterobactin Siderophore |
71 nt |
80°C |
GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATG |
P074 |
Ent E Biosynthesis of Enterobactin Siderophore |
43 nt |
81°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG |
P075 |
Ent F Biosynthesis of Enterobactin Siderophore |
68 nt |
75°C |
GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCA |
P076 |
Ent F Biosynthesis of Enterobactin Siderophore |
47 nt |
78°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG |
P077 |
RBS sfGFP - Plasmid 3 |
65 nt |
76°C |
CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAA |
P078 |
RBS sfGFP - Plasmid 3 |
58 nt |
75°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG |
P079 |
Promoteur Lac + Lac Operator |
93 nt |
82°C |
GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAG |
P080 |
Promoteur Lac + Lac Operator |
93 nt |
82°C |
GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGT |
P081 |
RBS positive control - Plasmid 2 |
46 nt |
77°C |
CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA |
P082 |
RBS positive control - Plasmid 2 |
46 nt |
77°C |
TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG |
P083 |
Lac I - Plasmid 1/2 |
40 nt |
72°C |
GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC |
P084 |
Lac I - Plasmid 1/2 |
56 nt |
76°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG |