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Team:DTU-Denmark/Notebook/7 August 2013
From 2013.igem.org
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(→Gel on yesterdeys PCR) |
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*Nir 5uL MgCl2 | *Nir 5uL MgCl2 | ||
*Nir 20uL MgCl2 | *Nir 20uL MgCl2 | ||
| - | *12 | + | *12 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, x7 polymerase |
| - | *13 | + | *13 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel |
| - | *14 | + | *14 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel |
| - | *15 | + | *15 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase |
| - | *16 | + | *16 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase |
| - | *7 | + | *7 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase |
| - | *8 | + | *8 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase |
| - | *9 | + | *9 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase |
| - | *10 | + | *10 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase |
| - | *11 | + | *11 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, Phusion polymerase |
[[File:2013-08-07 ara nir2.jpg| 600px]] | [[File:2013-08-07 ara nir2.jpg| 600px]] | ||
Revision as of 11:57, 7 August 2013
7 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Results
Gel on yesterdays PCR
ara, SPL is from yesterdays PCR with 5% DMSO, Nir and the sample numbers is also from yesterday, here the composition can be seen.
- ara
- ara
- SPL
- SPL
- Nir 0 MgCl2
- Nir 1uL MgCl2
- Nir 5uL MgCl2
- Nir 20uL MgCl2
- 12 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, x7 polymerase
- 13 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel
- 14 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel
- 15 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase
- 16 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase
- 7 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase
- 8 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase
- 9 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase
- 10 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase
- 11 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, Phusion polymerase
Conclusion
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