"
Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 2nd July.html
From 2013.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
</br></em></b></p> | </br></em></b></p> | ||
<!-- === Modify from here === --> | <!-- === Modify from here === --> | ||
| - | Plasmid Extraction | + | Plasmid Extraction<br> |
| - | + | ||
Plasmids pD004 (pCDF.ew12 zmFNR Spectinomycin), pD005 (pACYC.ew13 soFD, zmSIR Chloramphenicol), pD006 (pACYC.ew17 zmSIR Chloramphenicol) were extracted from NEBTurbo cells using a Thermo Scientific GeneJet Plasmid mini prep kit as described in protocol (). Lacking Resuspention solution we used some from a different mini prep upstairs. Plasmids were eluted in 100ul of nanopure water and frozen at -20ºC. | Plasmids pD004 (pCDF.ew12 zmFNR Spectinomycin), pD005 (pACYC.ew13 soFD, zmSIR Chloramphenicol), pD006 (pACYC.ew17 zmSIR Chloramphenicol) were extracted from NEBTurbo cells using a Thermo Scientific GeneJet Plasmid mini prep kit as described in protocol (). Lacking Resuspention solution we used some from a different mini prep upstairs. Plasmids were eluted in 100ul of nanopure water and frozen at -20ºC. | ||
<!-- === To here === --> | <!-- === To here === --> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 14:25, 9 August 2013
Drug Screening
ASDFDay Numbersuffix Month
MiniPrep: Plasmids pD004, pD005 and pD006 were extracted from NEBturbo vectors
Plasmid ExtractionPlasmids pD004 (pCDF.ew12 zmFNR Spectinomycin), pD005 (pACYC.ew13 soFD, zmSIR Chloramphenicol), pD006 (pACYC.ew17 zmSIR Chloramphenicol) were extracted from NEBTurbo cells using a Thermo Scientific GeneJet Plasmid mini prep kit as described in protocol (). Lacking Resuspention solution we used some from a different mini prep upstairs. Plasmids were eluted in 100ul of nanopure water and frozen at -20ºC.
