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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog
From 2013.igem.org
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{{TeamBYUProvo}} | {{TeamBYUProvo}} | ||
| - | + | '''5/1/13''' | |
- Commencement of spring!!! | - Commencement of spring!!! | ||
| Line 7: | Line 7: | ||
- Discussed goals and outlined plans for spring term | - Discussed goals and outlined plans for spring term | ||
| - | + | '''5/2/13''' | |
- Designed primers for amplifying and sequencing phage capsid protein | - Designed primers for amplifying and sequencing phage capsid protein | ||
| Line 13: | Line 13: | ||
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly | - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly | ||
| - | + | '''5/3/13''' | |
- Performed agar test, focusing primarily on ×8 | - Performed agar test, focusing primarily on ×8 | ||
| Line 23: | Line 23: | ||
: [[5.3 T7 phage amplification/purification]] | : [[5.3 T7 phage amplification/purification]] | ||
| - | + | '''5/4/13''' | |
- Performed dilution series using stock 5.3 (-1 through -11) | - Performed dilution series using stock 5.3 (-1 through -11) | ||
| Line 29: | Line 29: | ||
- Started two 5mL overnights of BL21 | - Started two 5mL overnights of BL21 | ||
| - | + | '''5/5/13''' | |
- Spot test using stock 5.3 and its dilution series (from 5.4) | - Spot test using stock 5.3 and its dilution series (from 5.4) | ||
| Line 43: | Line 43: | ||
: [[5.5 Amplification from a plaque test]] | : [[5.5 Amplification from a plaque test]] | ||
| - | + | '''5/6/13''' | |
- Continued [[5.3 T7 phage amplification/purification]] | - Continued [[5.3 T7 phage amplification/purification]] | ||
| Line 51: | Line 51: | ||
: [[5.6 T7+ Liquid Culture Phage Concentration Test]] | : [[5.6 T7+ Liquid Culture Phage Concentration Test]] | ||
| - | + | '''5/7/13''' | |
- Started two 5mL of E coli BL21 overnight | - Started two 5mL of E coli BL21 overnight | ||
| Line 57: | Line 57: | ||
- Designed procedure for applying mutagen and selecting for T7 | - Designed procedure for applying mutagen and selecting for T7 | ||
| - | + | '''5/8/13''' | |
- Went over procedure for applying mutagen and PCR with Dr. Grose | - Went over procedure for applying mutagen and PCR with Dr. Grose | ||
| Line 67: | Line 67: | ||
- Learned how to create LB plates | - Learned how to create LB plates | ||
| - | + | '''5/9/13''' | |
- Practiced with ×6 and ×8 top agar | - Practiced with ×6 and ×8 top agar | ||
Revision as of 15:24, 24 May 2013
5/1/13
- Commencement of spring!!!
- Discussed goals and outlined plans for spring term
5/2/13
- Designed primers for amplifying and sequencing phage capsid protein
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13
- Performed agar test, focusing primarily on ×8
- Processed phage amplification
5/4/13
- Performed dilution series using stock 5.3 (-1 through -11)
- Started two 5mL overnights of BL21
5/5/13
- Spot test using stock 5.3 and its dilution series (from 5.4)
- Started liquid culture for purification team (at around noon)
- 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
- Started 5.5 amplification from a plaque test
5/6/13
- Continued 5.3 T7 phage amplification/purification
- Performed liquid culture phage concentration test
5/7/13
- Started two 5mL of E coli BL21 overnight
- Designed procedure for applying mutagen and selecting for T7
5/8/13
- Went over procedure for applying mutagen and PCR with Dr. Grose
- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test
- Started two 5mL BL21 overnights
- Learned how to create LB plates
5/9/13
- Practiced with ×6 and ×8 top agar
- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2
- Discussed plans and schedule for next week.
