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13/08/13
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==Making agar, chloroamphenicol and chloroamphenicol/IPTG plates== | ==Making agar, chloroamphenicol and chloroamphenicol/IPTG plates== | ||
| + | *One container of 400 ml Luria Agar was melted down in the microwave. This is enough to make 20 agar petri dishes. | ||
| + | *This was poured straight into 4 petri dishes to make agar plates | ||
| + | *The remaining agar had 0.64 ul of chloroamphenicol added to it (concentration = 24 ug/ml), and was mixed thoroughly | ||
| + | *The resulting solution was poured into 4 petri dishes, to make 4 chloroamphenicol agar plates | ||
| + | *The remaining agar solution had 1.5 ul of IPTG added to it (concentration = 24 ug/ml) | ||
| + | *This was mixed thoroughly, and then used to make up the remaining 12 chloroamphenicol/IPTG plates. | ||
Revision as of 15:39, 13 August 2013
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Making agar, chloroamphenicol and chloroamphenicol/IPTG plates
- One container of 400 ml Luria Agar was melted down in the microwave. This is enough to make 20 agar petri dishes.
- This was poured straight into 4 petri dishes to make agar plates
- The remaining agar had 0.64 ul of chloroamphenicol added to it (concentration = 24 ug/ml), and was mixed thoroughly
- The resulting solution was poured into 4 petri dishes, to make 4 chloroamphenicol agar plates
- The remaining agar solution had 1.5 ul of IPTG added to it (concentration = 24 ug/ml)
- This was mixed thoroughly, and then used to make up the remaining 12 chloroamphenicol/IPTG plates.
