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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog
From 2013.igem.org
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| - | '''5/1/13''' | + | <br> |
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| + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage Spring Notebook: April 29 - May 12 Daily Log'''</font> | ||
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| + | | style="width: 18%; background-color: transparent;"| | ||
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| + | <font color="#333399" size="3" font face="Calibri"> | ||
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| + | Overview | ||
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| + | [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|Winter]] | ||
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| + | [[Team:BYU Provo/Notebook/SmallPhage/Springexp|Spring]] | ||
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| + | [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|Summer]] | ||
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| + | [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|Fall]] | ||
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| + | </font> | ||
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| + | | style="width: 82%; background-color: transparent;"| | ||
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| + | <font face="Calibri" size="3"> | ||
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| + | <font size="4"> '''5/1/13''' </font> | ||
- Commencement of spring!!! | - Commencement of spring!!! | ||
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- Discussed goals and outlined plans for spring term | - Discussed goals and outlined plans for spring term | ||
| - | '''5/2/13''' | + | <br> |
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| + | <font size="4"> '''5/2/13''' </font> | ||
- Designed primers for amplifying and sequencing phage capsid protein | - Designed primers for amplifying and sequencing phage capsid protein | ||
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- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly | - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly | ||
| - | '''5/3/13''' | + | <br> |
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| + | <font size="4"> '''5/3/13''' </font> | ||
- Performed agar test, focusing primarily on ×8 | - Performed agar test, focusing primarily on ×8 | ||
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: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] | ||
| - | '''5/4/13''' | + | <br> |
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| + | <font size="4"> '''5/4/13''' </font> | ||
- Performed dilution series using stock 5.3 (-1 through -11) | - Performed dilution series using stock 5.3 (-1 through -11) | ||
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- Started two 5mL overnights of BL21 | - Started two 5mL overnights of BL21 | ||
| - | '''5/5/13''' | + | <br> |
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| + | <font size="4"> '''5/5/13''' </font> | ||
- Spot test using stock 5.3 and its dilution series (from 5.4) | - Spot test using stock 5.3 and its dilution series (from 5.4) | ||
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: [[5.5 Amplification from a plaque test]] | : [[5.5 Amplification from a plaque test]] | ||
| - | '''5/6/13''' | + | <br> |
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| + | <font size="4"> '''5/6/13''' </font> | ||
- Continued [[5.3 T7 phage amplification/purification]] | - Continued [[5.3 T7 phage amplification/purification]] | ||
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: [[5.6 T7+ Liquid Culture Phage Concentration Test]] | : [[5.6 T7+ Liquid Culture Phage Concentration Test]] | ||
| - | '''5/7/13''' | + | <br> |
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| + | <font size="4"> '''5/7/13''' </font> | ||
- Started two 5mL of E coli BL21 overnight | - Started two 5mL of E coli BL21 overnight | ||
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- Designed procedure for applying mutagen and selecting for T7 | - Designed procedure for applying mutagen and selecting for T7 | ||
| - | '''5/8/13''' | + | <br> |
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| + | <font size="4"> '''5/8/13''' </font> | ||
- Went over procedure for applying mutagen and PCR with Dr. Grose | - Went over procedure for applying mutagen and PCR with Dr. Grose | ||
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- Learned how to create LB plates | - Learned how to create LB plates | ||
| - | '''5/9/13''' | + | <br> |
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| + | <font size="4"> '''5/9/13''' </font> | ||
- Practiced with ×6 and ×8 top agar | - Practiced with ×6 and ×8 top agar | ||
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- Discussed plans and schedule for next week. | - Discussed plans and schedule for next week. | ||
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| + | </font> | ||
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| + | |} | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} | ||
Revision as of 15:55, 27 May 2013
| Small Phage Spring Notebook: April 29 - May 12 Daily Log
| ||
|
Overview
|
5/1/13 - Commencement of spring!!! - Discussed goals and outlined plans for spring term
5/2/13 - Designed primers for amplifying and sequencing phage capsid protein - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13 - Performed agar test, focusing primarily on ×8 - Processed phage amplification
5/4/13 - Performed dilution series using stock 5.3 (-1 through -11) - Started two 5mL overnights of BL21
5/5/13 - Spot test using stock 5.3 and its dilution series (from 5.4) - Started liquid culture for purification team (at around noon)
- Started 5.5 amplification from a plaque test
5/6/13 - Continued 5.3 T7 phage amplification/purification - Performed liquid culture phage concentration test
5/7/13 - Started two 5mL of E coli BL21 overnight - Designed procedure for applying mutagen and selecting for T7
5/8/13 - Went over procedure for applying mutagen and PCR with Dr. Grose - Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test - Started two 5mL BL21 overnights - Learned how to create LB plates
5/9/13 - Practiced with ×6 and ×8 top agar - Started 5.9 T7+ Liquid Culture Phage Concentration Test #2 - Discussed plans and schedule for next week.
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