Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/PR
From 2013.igem.org
(Difference between revisions)
Line 41: | Line 41: | ||
:: - phage: 4mL LB + 1mL E. coli overnight | :: - phage: 4mL LB + 1mL E. coli overnight | ||
- | : [[File:SpringPR1P1.JPG| | + | : [[File:SpringPR1P1.JPG|400px|center]] |
:: Obvious clearage was seen for the + phage test tube | :: Obvious clearage was seen for the + phage test tube | ||
: Spot test result | : Spot test result | ||
:: We purified the phage via centrifugation and adding chloroform. This batch of phage is stored as stock 5.3 in the fridge. | :: We purified the phage via centrifugation and adding chloroform. This batch of phage is stored as stock 5.3 in the fridge. | ||
- | : [[File:SpringPR1P2.JPG| | + | : [[File:SpringPR1P2.JPG|400px|center]] |
:: This confirmed our conclusion that we are able to grow T7 in liquid culture with E. coli BL21. | :: This confirmed our conclusion that we are able to grow T7 in liquid culture with E. coli BL21. | ||
<br> | <br> |
Revision as of 16:58, 27 May 2013
Small Phage Spring Notebook: April 29 - May 12 Progress Report
| ||
Overview
|
1. Goals for the week
2.Experiments and results Phage growth in liquid culture (5.3 Phage Amplification/Purification)
Amplifying phage (5.6 T7+ Liquid Culture Phage Concentration Test)
Selection test
Stabbing test (5.5 Amplification from a plaque test)
Others
3. Next Steps
|