Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/PR
From 2013.igem.org
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: We wanted to see whether or not we can grow liquid culture from a single plaque. | : We wanted to see whether or not we can grow liquid culture from a single plaque. | ||
:: 4mL LB + 1mL E. coli overnight + 1 stab at a plaque from a ×6 plate in the 5.3 T7 Phage Selection Method Test. The plaque in question is circled in the photo above. After incubation for approximately 24 hours, phage solution was purified and spot test was performed. The remaining phage solution is labeled stock 5.4 in the fridge. | :: 4mL LB + 1mL E. coli overnight + 1 stab at a plaque from a ×6 plate in the 5.3 T7 Phage Selection Method Test. The plaque in question is circled in the photo above. After incubation for approximately 24 hours, phage solution was purified and spot test was performed. The remaining phage solution is labeled stock 5.4 in the fridge. | ||
- | : [[File: | + | : [[File:5.4_stock_spot_test.JPG|400px|center]] |
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Revision as of 17:07, 27 May 2013
Small Phage Spring Notebook: April 29 - May 12 Progress Report
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Overview
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1. Goals for the week
2.Experiments and results Phage growth in liquid culture (5.3 Phage Amplification/Purification)
Amplifying phage (5.6 T7+ Liquid Culture Phage Concentration Test)
Selection test
Stabbing test (5.5 Amplification from a plaque test)
Others
3. Next Steps
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